New TentaGel XV (eXtended swelling Volume)

Published on 03/12/2013

Resin especially designed for the synthesis of long and difficult peptide sequences!

Due to modifications of the polystyrene backbone, TentaGel XV shows extended swelling properties providing a large reaction volume. It is a superior resin for synthesizing difficult sequences, aggregating peptides, mini proteins, and PNAs in high purity and excellent yields.

The dedicated crosslinking design in TentaGel XV leads to increased swelling, which is similar or even higher compared to PEGA^® or ChemMatrix^® resins. The polar PEG spacers allow the composite resin to swell in a wide range of solvents including water, methanol, DMF, DCM and make its handling very easy.

The high swelling creates an extended reaction space providing ideal conditions for the synthesis of difficult sequences, aggregating peptides, mini proteins and PNAs in high purities and excellent yields.

mClaudin (53-81) 29mer SSVSQSTGQI QSKVEDSLLN LNSTLQATR-NH2 | Purified yield of PNA ₄-peptide ₁₅-PNA ₁₁

Synthesis guidelines for 1g TentaGel XV resin in DMF

1g resin needs app. 20 ml of DMF
Filtering: app. 6 ml of DMF remains in the resin

Coupling to the dry resin:
add 20 ml of a 0.2 molar solution of activated compound

Coupling to the DMF swollen resin:
add 14 ml of a 0.3 molar solution of activated compound
the resulting coupling concentration is app. 0.2 molar

(14 ml 0.3 molar solution + 6 ml DMF remaining from the resin = 20 ml total solvent volume)
Washing: use 8-10 washing/filtering cycles with 14 ml of DMF each

References:

R. Pipkorn et al., Int. J. Med. Sci. 2013; 10: 331.
W. Rapp et al. in Peptides 2012, Proceedings of the 32nd European Peptide Symposium, G.Kokotos, V.Constantinou-Kokotos, J.Matsoukas (Eds.), European Peptide Society, 2012; p.28.
S. Rawer et al. in Peptides 2012, Proceedings of the 32nd European Peptide Symposium, G.Kokotos, V.Constantinou-Kokotos, J.Matsoukas (Eds.), European Peptide Society, 2012; p.406.