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BSSS
Chemical name: Bis(sulfosuccinimidyl) suberate sodium salt // Synonyms: Sulfo-DSS, BS3, Suberic acid bis(3-sulfo-N-hydroxysuccinimide ester) sodium salt; Component: CAS 127634-19-9
- Product code:RL-2770
- CAS No.:127634-19-9
- Formula:C16H18N2Na2O14S2
- Storage temperature:-20°C
- Molecular weight:572,43 g/mol
from €350.00
description
This molecule carries amino reactive Sulfo-NHS esters on both ends and is a water soluble, homo-bifunctional protein cross-linker (spacer length: 11.4 Å). Due to its water solubility, conjugation reactions conveniently can take place at physiological conditions. This 8-atom spacer is non-cleavable and the molecule is not cell membrane permeable. It can be used to prepare antibody-protein conjugates, for crosslinking cell surface proteins, and for covalently orienting antibody to an immobilized Protein A or Protein G resin.
General BSSS Cross-linking Protocol:
1. Allow vial of BSSS to fully equilibrate to ambient temperature before opening to prevent condensation inside the vial (BS3 is moisture-sensitive).
2. Immediately before use, prepare a 50mM solution of BSSS, by dissolving 10 mg BSSS in 350 ìL of 25 mM Sodium Phosphate, pH 7.4 (do not use amine containing buffers for the conjugation reaction).
3. Add BSSS solution (20-fold excess cross-linker to protein) to the protein sample so that the final concentration is between 0.5 to 5 mM.
4. Allow the sample to react at room temperature for 45 minutes to 1 hour. Allow slightly longer if reaction must be done on ice (this reaction rate is only slightly slower at low temperatures).
5. Quench and unreacted BSSS with 25 mM to 60 mM Tris and allow to react for 10-15 minutes at room temperature.
6. Desalt sample to remove unreacted BSSS, i.e. gel filtration, dialysis, etc.
references
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Mapping the Topology and Determination of a Low-Resolution Three-Dimensional Structure of the Calmodulin−Melittin Complex by Chemical Cross-Linking and High-Resolution FTICRMS: Direct Demonstration of Multiple Binding Modes. Daniela M. Schulz, Christian Ihling, G. Marius Clore, Andrea Sinz; Biochemistry 2004; 43(16): 4703-4715. DOI: org/10.1021/bi036149f
Selective Inactivation of Adrenomedullin over Calcitonin Gene-related Peptide Receptor Function by the Deletion of Amino Acids 14-20 of the Mouse Calcitonin-like Receptor. Daniela Koller, Lars M. Ittner, Roman Muff, Knut Husmann, Jan A. Fischer, Walter Born; J. Biol. Chem. 2004; 279: 20387-20391. DOI: 10.1074/jbc.M313058200
Mactinin, a fragment of cytoskeletal alpha-actinin, is a novel inducer of heat shock protein (Hsp)-90 mediated monocyte activation; Sharon D Luikart, Angela Panoskaltsis-Mortari, Timothy Hinkel, Robert T Perri, Kalpna Gupta, Theodore R Oegema, Pankaj Gupta; BMC Cell Biology 2009; 10: 60. DOI: org/10.1186/1471-2121-10-60
The program for processing newly synthesized histones H3.1 and H4; Eric I. Campos, Jeffrey Fillingham, Guohong Li, Haiyan Zheng, Philipp Voigt, Wei-Hung W Kuo, Harshika Seepany, Zhonghua Gao, Loren A Day, Jack F Greenblatt, Danny Reinberg; Nature Structural & Molecular Biology 2010; 17(11): 1343-1351. DOI: org/10.1038/nsmb.1911
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Mps1 directs the assembly of Cdc20 inhibitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling; John Maciejowski, Kelly A. George, Marie-Emilie Terret, Chao Zhang, Kevan M. Shokat, Prasad V. Jallepalli; J. Cell. Biol. 2010; 190(1): 89-100. DOI: org/10.1083/jcb.201001050
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