Welcome to Iris Biotech
For better service please confirm your country and language we detected.
Currency
EUR
Contact rapide
Veuillez m'envoyer plus d'informations sur
H-L-Ala-AMC
Nom chimique: L-Alanine-7-amido-4-methylcoumarin // Synonymes: (S)-2-amino-N-(4-methyl-2-oxo-2H-chromen-7-yl)propanamide, L-Alanine 4-methylcoumaryl-7-amide, Ala-7-amino-4-methylcoumarin, H-Ala-AMC, Ala-AMC
- n° Art.:HAA1200
- n° CAS:77471-41-1
- Formule:C13H14N2O3
- Storage temperature:2-8°C
- Masse moléculaire:246,27 g/mol
- Pureté:min. 99%
- Pureté Énantiomérique:min. 99,7%
from 60,00 €
description
HAA1200 is a fluorogenic substrate for Aminopeptidase M, which is a metalloprotease that hydrolyzes N-terminal amino acids. The substrate shows a weak fluorescence (absorption/emission ~ 330/390 nm). After enzymatic cleavage, the fluorescence of the end product (AMC) is drastically increased (absorption/emission ~ 340/440 nm). AMC combines a high fluorescence yield with a large Stokes shift and is relatively photostable.
references
Synthesis of a Statistically Exhaustive Fluorescent Peptide Substrate Library for Profiling Protease Specificity; J. E. Sheppeck II, H. Kar, L. Gosink, J. B. Wheatley, E. Gjerstad, S. M. Loftus, A. R. Zubiria, J. W. Janc; Bioorg. Med. Chem. Lett. 2000, 10: 2639-2642. https://doi.org/10.1016/S0960-894X(00)00545-X.
Synthesis and Fluorogenic Properties of Some 1-(Coumarin-7-yl)-4,5-dihydro-1,2,4-triazin-6(1H)-ones; M. S. Mustafa, M. M. El-Abadelah, M. S. Mubarak; Int. J. Chem. 2011, 3(4): 89-103. https://doi.org/10.5539/ijc.v3n4p89.
Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries; J. L. Harris, B. J. Backes, F. Leonetti, S. Mahrus, J. A. Ellman, C. S. Craik; Proc. Natl. Acad. Sci. USA. 2000, 97(14): 7754-7759. https://doi.org/10.1073/pnas.140132697.
A Simple Fluorescent Labeling Method for Studies of Protein Oxidation, Protein Modification, and Proteolysis; A. M. Pickering, K. J. A. Davies; Free Radic Biol Med. 2012, 52(2): 239-246. https://doi.org/10.1016/j.freeradbiomed.2011.08.018.
* Disclaimer
We refer to our general terms and conditions.
All information contained in our webshop is compiled to the best of our knowledge.
Prices and product availability are subject to change and require reconfirmation. Please contact us for further information.
All information contained in our webshop is compiled to the best of our knowledge.
Prices and product availability are subject to change and require reconfirmation. Please contact us for further information.
Besoin d'informations supplémentaires sur ce produit?
entrer en contact
Brochures et dépliants
Des produits qui pourraient également vous intéresser!
