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A sensitive and quantitative method for the estimation of reactive metabolite formation in vitro utilizes dansyl labeled reduced glutathione (GSH) as a trapping agent and fluorescent detection for quantitation.
Dansyl Glutathione as a Trapping Agent for the Quantitative Estimation and Identification of Reactive Metabolites; Jinping Gan, Timothy W. Harper, Mei-Mann Hsueh, Qinling Qu, and W. Griffith Humphreys; Chem Res Toxicol. 2005; 18(5): 896-903. DOI: 10.1021/tx0496791.
An optimized method for determination of intracellular glutathione in mouse macrophage cultures by fluorimetric high-performance liquid chromatography; Hiroyoshi Komatsu, Fumiya Obata; Biomedical Chromatography 2003; 17(5): 345–350. DOI: 10.1002/bmc.250 .
Drug Metabolism and Pharmacokinetics Quick Guide; Siamak Cyrus Khojasteh, Harvey Wong, Cornelis E.C.A. Hop; Springer Science+Business Media 2011, ISBN 978-1-4419-5629-3. DOI: 10.1007/978-1-4419-56-3_1.
