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Halo-PEG(2)-NH2*HCl
Nom chimique: 12-Chloro-3,6-dioxa-dodecan-1-amine hydrochloride // Synonymes: 2-(2-((6-chlorohexyl)oxy)ethoxy)ethanamine hydrochloride, net-CAS 744203-60-9
- n° Art.:RL-3680
- n° CAS:1035373-85-3
- Formule:C10H22ClNO2*HCl
- Storage temperature:-20°C
- Masse moléculaire:223,74*36,46 g/mol
from 300,00 €
description
This chloroalkane linker serves as ligand for a specific modified bacterial haloalkane dehalogenase. This is a reporter protein whose sequence can easily be fused to a gene of interest. The reaction between the chloroalkane linker and the enzyme is fast and irreversible under physiological conditions and the system has been used extensively to measure protein-protein interactions in cells.
references
HaloTag technology: a versatile platform for biomedical applications; C. G. England, H. Luo and W. Cai; Bioconjug Chem 2015; 26: 975-86. https://doi.org/10.1021/acs.bioconjchem.5b00191.
HaloTag: a novel protein labeling technology for cell imaging and protein analysis; G. V. Los, L. P. Encell, M. G. McDougall, D. D. Hartzell, N. Karassina, C. Zimprich, M. G. Wood, R. Learish, R. F. Ohana, M. Urh, D. Simpson, J. Mendez, K. Zimmerman, P. Otto, G. Vidugiris, J. Zhu, A. Darzins, D. H. Klaubert, R. F. Bulleit and K. V. Wood; ACS Chem Biol 2008; 3: 373-382. https://doi.org/10.1021/cb800025k.
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