Name: OPSS-SNAP
SKU: RL-3990
Availability: InStock

Grouped product items

Nombre

Unité de vente

Prix

SKU

Disponibilité

5 mg

200,00 €

RL-3990.0005

25 mg

800,00 €

RL-3990.0025

Fiches de données de sécurité

description

Pyridyl disulfide conjugated benzylguanine (BG). The BG moiety reacts specifically and rapidly with the so-called SNAP-tag, a polypeptide protein tag, allowing irreversible and covalent labeling of SNAP fusion proteins with a disulfide linker.

Pyridyl disulfides undergo a disulfide exchange reaction with sulfhydryl groups to form disulfide bonds over a broad pH range also suitable for physiological pH. During the reaction, a disulfide exchange occurs between the biomolecule’s thiol group and the reagent’s 2-pyridyldithiol group. As a result, pyridine-2-thione is released, which can be followed spectrophotometrically (ëmax = 343 nm) to monitor the progress of the reaction.

references

Conditional and Reversible Activation of Class A and B G Protein-Coupled Receptors Using Tethered Pharmacology; T. Podewin, J. Ast, J. Broichhagen, N. H. F. Fine, D. Nasteska, P. Leippe, M. Gailer, T. Buenaventura, N. Kanda, B. J. Jones, C. M’Kadmi, J.-L. Baneres, J. Marie, A. Tomas, D. Trauner, A. Hoffmann-Röder, D. J. Hodson; ACS Cent. Sci. 2018; 4(2): 166-179. https://doi.org/10.1021/acscentsci.7b00237

Disulfide-Based Self-Immolative Linkers and Functional Bioconjugates for Biological Applications; Z. Deng, J. Hu and S. Liu; Macromol Rapid Commun 2020; 41: e1900531. https://doi.org/10.1002/marc.201900531

* Disclaimer

We refer to our general terms and conditions.
All information contained in our webshop is compiled to the best of our knowledge.
Prices and product availability are subject to change and require reconfirmation. Please contact us for further information.