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Biotin-SNAP
Chemischer Name: N-(4-(((2-amino-9H-purin-6-yl)oxy)methyl)benzyl)-6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamide // Synonyme: Biotin-BG, Biotin-Benzylguanine
- Art-Nr.:RL-3860
- CAS Nr.:471918-16-8
- Formel:C29H39N9O4S
- Lagertemperatur:-20°C
- Molare Masse:609,75 g/mol
Ab 200,00 €
description
Biotin conjugated benzylguanine (BG). The BG moiety reacts specifically and rapidly with the so-called SNAP-tag, a polypeptide protein tag, allowing irreversible and covalent labeling of SNAP fusion proteins with biotin. Biotinylated proteins can for example be selectively isolated based on the high affinity towards avidin representing a useful tool for purification, immobilization, and labeling.
references
A general method for the covalent labeling of fusion proteins with small molecules in vivo; A. Keppler, S. Gendrezig, T. Gronemeyer, H. Pick, H. Vogel, K. Johnsson; Nat. Biotechnol. 2003; 21: 86-89. https://doi.org/10.1038/nbt765.
Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo; A. Juillerat, T. Gronemeyer, A. Keppler, S. Gendreizig, H. Pick, H. Vogel, K. Johnsson; Chem. Biol. 2003; 10(4): 313-317. https://doi.org/10.1016/s1074-5521(03)00068-1.
Site-specific, Covalent Labeling of Recombinant Antibody Fragments via Fusion to an Engineered Version of 6-O-Alkylguanine DNA Alkyltransferase; F. Kampmeier, M. Ribbert, T. Nachreiner, S. Dembski, F. Beaufils, A. Brecht, S. Barth; Bioconjugate Chem. 2009; 20(5): 1010-1015. https://doi.org/10.1021/bc9000257.
SNAP-Tag Technology: A Useful Tool to Determine Affinity Constants and Other Functional Parameters of Novel Antibody Fragments; J. Niesen, M. Sack, M. Seidel, R. Fendel, S. Barth, R. Fischer, C. Stein; Bioconjugate Chem. 2016; 27(8): 1931-1941. https://doi.org/10.1021/acs.bioconjchem.6b00315.
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