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Continue to Iris Biotech GmbHSend request to US distributorPublished on 09.11.2011
Product of the month: February
H-L-Aha-OH*HCl 4-Azido-L-homoalanine Formula: C4H8N4O2*HCl CAS: 942518-29-8 CODE: HAA5730 1g: EUR 200,- US$ 300,- Prices are in EUR, net, exw Germany, shipping not include |
Recombinant Incorporation of Amino Acids into Proteins:
Mutant or modified aminoacyl-tRNA synthetases (aaRS) have been used to charge non-natural amino acids to the corresponding tRNA, which incorporates them into polypeptides or proteins during recombinant synthesis. As azido homoalanine (Aha) is a structure analogue of methionine (Met), Met has been replaced effectively by Aha in proteins by the native methionyl tRNA synthetase of E. coli, [1]. A modified yeast phenylalanyl-tRNA synthetase was prepared by introduction of a T415G or T415A mutation in its α-subunit; specific residues in tryptophanyl-tRNA synthetase and methionyl-tRNA synthetase are also identified, where mutagenesis results in specificity for non-natural aminoacyl-tRNA molecules. Further developments and inventions have been done to specifically incorporate a vast number of Click components into practically any protein.
Once the azido function has been built into the protein sequence, conjugation with a large number of diverse partners including PEG-polymers, dyes, cofactors, antibodies, small molecules, toxins, additional proteins and peptides opens a wide field with many different applications from therapeutics to diagnostics.
For Incorporation of Aha by Chemical Synthesis into Peptides or other Organic Molecules the following Building Blocks are available:
References: