Search results for: 'biotinyl'

PotM: Furan-Crosslinking and 5HP2O as Maleimide Alternative

Game Changer for Protein Modification: Discover oxidation-induced furan crosslinking and 5-hydroxy-1,5-dihydro-2H-pyrrol-2-ones ( 5HP2O s) as stable alternative to maleimides.

2-Cyanopyridines for Click-Like Reactions

Have an additional ace up your sleeve for selective and efficient peptide modification, cyclization, and/or bioconjugation. Explore 2-cyanopyridines for click-like reactions. Get more information!

Review – Product Highlights 2023 (Part 1)

Curious about our latest portfolio additions from January to June 2023? Discover our New Products Flyer and check out the summary of recent product highlights! Get in contact for more details!

PotM: Functionalized Dextrans

Our monofunctionalized dextrans are enriching the toolbox of polymer therapeutics – Dextrans benefit of excellent solubility, biocompatibility, biodegradability, and non-immunogenicity.

PotM: Biotin – Desthiobiotin - Iminobiotin

Herein, we present 2-Iminobiotin, a cyclic guanidino analogue of biotin with pH-dependent affinity for avidin, and compare it to biotin and desthiobiotin. Click here for more detailed information.

PotM: Substrates for Fusion (Halo/Snap/Clip)-Tagged Proteins

Discover our selection of substrates for Halo-, Snap-, and Clip-tagged proteins bearing different terminal groups suitable for further functionalization, e.g. biotinylation and Click chemistry.

PotM: Functionalized Biotinylation Reagents

Iris Biotech offers a variety of (functionalized) biotin and desthiobiotin reagents reactive towards certain functional groups. Discover our growing portfolio and latest additions.

Cleavable Bifunctional Biotin-Linkers

Biotinylation of proteins and their purification via Streptavidin beads is a widely applied method.

Product of the Month: Biotin-Aniline for Proximity Labeling of RNA

Proximity labeling of proteins with biotin-phenol (biotin-tyramide) and an engineered peroxidase enzyme (e.g. APEX = Engineered Ascorbate Peroxidase) is a pivotal tool for molecular biology.

Membrane-Impermeant Biotin Tyramide

Methods for proteomic mapping of cellular organelles or subdomains that avoid the necessity of cell lysis and purification by targeting specific subcellular regions have several advantages.

Targeted and Reversible Biotinylation with Biotin-SS-Tyramide

Our new Biotin-SS-Tyramide linker is a valuable tool for peroxidase-promoted targeted protein biotinylation. By using peroxidase-tagged antibodies, proteins and protein clusters can be selectively biotinylated, and then isolated using streptavidin. The biotin tag can be subsequently removed using reducing agents (e.g. glutathione).

Benzotriazole Amino Acids

Benzotriazol activated carboxylic acids: Fast reaction (within minutes) at room temperature with amines and other nucleophiles under addition of base (in both water and non- aqueous solvents). Stable to racemization when coupling with nucleophiles. High solubility in organic solvents like DMF and DCM. Stable in water.

Biotin Tyramide Reagents for Tyrosine-/Protein-Biotinylation

Tyramine compounds are converted to highly reactive radicals by horseradish peroxidase in presence of H2O2 which preferentially react with surface exposed tyrosines. Biotin Tyramide and Biotin-PEG-Tyramide therefore are ideal reagents used for tyrosine-/protein-biotinylation.