description

THPTA is a water soluble alternative to TBTA (RL-2010) and highly efficient catalyst or ligand for click chemistry in partially organic and particularly in completely aqueous reactions. The benefits of a completely aqueous reaction include the biological labeling of live cells or the labeling of proteins without the concern of denaturing secondary structures. THPTA complexes Cu(I) hence is blocking the bioavailability of Cu(I) and ameliorating the potential toxic effects while maintaining the catalytic effectiveness in click conjugations. Successful Click reactions with oligonucleotids can be found in many publications.

references

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A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective “Ligation” of Azides and Terminal Alkynes; Vsevolod V. Rostovtsev, Luke G. Green, Valery V. Fokin, K.

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Oligonucleotide and Polymer Functionalized Nanoparticles for Amplification-Free Detection of DNA; D. A. C. Thomson, E. H. L. Tee, N. T. D. Tran, M. J. Monteiro and M. A. Cooper;

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