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Cleavable Linker - Dde-Helping Hands
Placing Dde [N-1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)-3-ethyl] as one terminal group of a linker and a functional group prone for conjugation as the other or using Dde as the central connective portion of a linker allows for the creation of new bifunctional linkers.
Cleavage conditions require a (buffered) aqueous solution of hydrazine or hydroxylamine thus being orthogonal to Fmoc/tBu protocols. Furthermore, the cleavage can be followed spectrophotometrically due to the formation of a chromophoric product.
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